Comparative analysis of antimicrobial efficacy and cytotoxicity of Terminalia arjuna bark extract mediated zinc oxide nanoparticles (ZnONPs) and selenium nanoparticles (SeNPs): An in-vitro study

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Original Article

Author Details : R Vijayalakshmi*, N Ambalavanan, Rajeshkumar Shanmugam, Jaideep Mahendra, Uma Sudhakar, Tharani Munusamy

Volume : 9, Issue : 4, Year : 2024

Article Page : 188-194

https://doi.org/10.18231/j.ijpi.2024.039

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Abstract

Background: This study provides an in-depth comparative analysis of the antimicrobial efficacy and cytotoxic effects of -mediated Selenium Nanoparticles (TA-SeNPs) and Zinc Oxide Nanoparticles (TA-ZnONPs) in the context of chronic periodontitis. The well diffusion assay and Brine Shrimp Lethality Assay were employed to assess antibacterial efficacy and cytotoxicity, respectively.
Aim & Objective: The aim of the studyis to contribute to the understanding of the potential applications and safety considerations associated with green-synthesized ZnONPs and SeNPs as a local drug delivery agent in the treatment of chronic periodontitis.The objective of the study is tocomparatively analyse the antimicrobial efficacy and cytotoxic effects of -mediated Selenium Nanoparticles (TA-SeNPs) and Zinc Oxide Nanoparticles (TA-ZnONPs).
Materials and Methods: Mueller Hinton agar was prepared for the well diffusion assay, and 24-hour-old cultures of , , and were swabbed onto the agar. Different concentrations (25, 50, 100 µg/mL) of TA-SeNPs and TA-ZnONPs were loaded into wells, and a plant extract served as the control. Plates were incubated at 37?C for 24 hours, and inhibition zones were measured. For the Brine Shrimp Lethality Assay, a saline solution was prepared, and TA-SeNPs and TA-ZnONPs were introduced at concentrations of 5, 10, 20, 40, and 80?g/mL Viability was observed after a 24-hour incubation.
Results: Employing antimicrobial activity against oral pathogens, SeNPs demonstrated a specific and heightened response against , while ZnONPs exhibited consistent efficacy against all tested pathogens, suggesting broad-spectrum potential. However, the Brine Shrimp Lethality Assay revealed SeNPs concentration-dependent cytotoxicity, necessitating careful dosage considerations. In contrast, ZnONPs displayed a more favorable cytotoxic profile.
Conclusion: These small differences underscore the importance of optimizing dosages to balance antimicrobial efficacy and safety, providing valuable details for the development of targeted nanotherapeutics for chronic periodontitis. Further mechanistic studies and in vivo investigations are needed to refine their potential applications in periodontal infection management.
 

Keywords: Chronic periodontitis, Local drug delivery, Green synthesis, Terminalia arjuna, SeNPs, ZnONPs, Antimicrobial activity, Cytotoxic effect

How to cite : Vijayalakshmi R, Ambalavanan N, Shanmugam R , Mahendra J, Sudhakar U, Munusamy T, Comparative analysis of antimicrobial efficacy and cytotoxicity of Terminalia arjuna bark extract mediated zinc oxide nanoparticles (ZnONPs) and selenium nanoparticles (SeNPs): An in-vitro study. IP Int J Periodontol Implantol 2024;9(4):188-194

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